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5.5. DIFFERENTIATION OF HUMAN GERM CELL CULTURES 5.5.1. In Vitro Assessment of Pluripotency In our experience, the VP germ cells/hEGCs have proven particularly prone to spontaneous differentiation in culture, despite strict maintenance of the conditions that effect their derivation. These dif culties have been alluded to by most of the groups that have reported on human germ cell culture [Turnpenny et al., 2003; Liu et al., 2004; Onyango et al., 2002]. However, during their early proliferation, when positive for undifferentiated markers, VP cultures provide material for the in vitro demonstration of pluripotency. Our practice has been to encourage cells to aggregate, either through maintained growth beyond con uence or in suspension culture. The concomitant withdrawal of LIF, FGF-2, and forskolin and the loss of feeder cell in uence has also been reported to promote differentiation, resulting in structures that share some similarity to

This was shown for tert-alkyl, norbomyl, and methylcyclohexyl cations. Siskin studied the hydrogenolysis of cycloalkanes (and alkanes) in HF-TaFs system with 200-psi (pounds per square inch; lb/in ) hydrogen 07 He was able to show acid-catalyzed hydrogenolysis (ring or chain cleavage) and hydrogenation leading to open chain alkanes:

Bill Moschella has contributed a number of Expert Tutorials on sound-related topics. His biographical information can be found (together with his sound advice) in 16, Optimizing Flash Sound for Export.

11

first successful epoxidation with H2-colloidal Pt-N-methylimidazole catalyzed by Mn(TPP)CI,328 several other reducing agents have been introduced,124 but only the Mn(TPP)CI-02-ascorbate I54 and the Mn(TPP)CI-Zn-imidazole I52 systems yield epoxides exclusively. Of other related systems, molybdenum(V) porphyrin exhibits very high stereoselectivity with tert-BuOOH as the oxygen source (97% cis-2-hexene oxide and 99% trans-2-hexene oxide from cis- and trans-2-hexene, respectively) 29 Nonporphyrin complexes of iron were found to be stereoselective in the epoxidation of stilbene isomers. Iron cyclam, a nonporphyrin iron complex, gives the corresponding cis and trans epoxides in epoxidation with H 20 2 30 Fe(acach, in contrast, yields the trans epoxide from both stilbene isomers 31

A full set of color gures for this chapter is provided on the Visual Mixing CD af xed to the back cover of the book. Handbook of Industrial Mixing: Science and Practice, Edited by Edward L. Paul, Victor A. Atiemo-Obeng, and Suzanne M. Kresta ISBN 0-471-26919-0 Copyright 2004 John Wiley & Sons, Inc.

15-11

1 0.238802 0.247853 0.271477 0.228364

[see the alternative form of (10.9) in part (a) of this exercise]. Impose this condition, demand that the coefficients of p and qv vanish separately] and use the definitions WI = -A1 and W2 = A2M2 to obtain (10.11).

+ 0.005

FIGURE 30-1

4.2.2.2 Differentiated Services DiffServ [7,8] is a QoS paradigm that provides a method for classifying services of several applications. This is done by providing different levels of service to different packets, by means of the DiffServ CodePoint (DSCP). DSCP are included as part of the header of each IP packet. In IPv4 the information is stored in the TOS field, while in IPv6 header the Traffic Class octet is used. The DiffServ QoS mechanisms are applied in edge routers, and consist of (Figure 4.4):

To create an instance of a value object class in MXML, follow these steps:

2.8

Oldshue, J. Y. (1983). Fluid Mixing Technology, McGraw-Hill, New York. Weetman, R. J. (1994). Development of an erosion resistant mixing impeller for large scale solid suspension applications with CFD comparisons, presented at the 8th European Conference on Mixing, Cambridge. Weetman, R. J. (1998a). Examining ow structures in a mixing vessel using computational uid dynamics, presented at the AIChE Annual Meeting, Miami Beach, FL. Weetman, R. J. (1998b). Automated sliding mesh CFD computations for uidfoil impellers, presented at the 9th European Conference on Mixing, Paris. Weetman, R. J., and J. Y. Oldshue (1988). Power, ow and shear characteristics of mixing impellers, presented at the 6th European Conference on Mixing, Pavia, Italy. Weetman, R. J., and R. N. Salzman (1981). Impact of side ow on mixing impeller, Chem. Eng. Prog., June.

Q = /d31j0(2) = Jd3x4t(x)$(2)

Table 3-6 (continued)

Figure 20-4: The diagram on the left illustrates a Web site that uses multiple HTML pages, each with an individual .SWF file. The diagram on the right shows a Web site that uses one HTML page (or frameset) that has one primary .SWF file, which loads other .SWF files as needed.