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The commercial use of animal and insect cells at scales greater than 10 000 L for the production of posttranslationally modi ed proteins in kilogram quantities and viral vaccines using recombinant DNA and cell fusion techniques has made cell culture a cornerstone of modern biotechnology. Cell culture is being used increasingly for the production of highly valuable biologicals such as viral vaccines, hormones, growth factors, enzymes, and monoclonal antibodies. The high cost of media can often result in the cell culture step becoming the most signi cant cost of the process. Hence, much work has been devoted to optimizing the culture media and feeding strategies for supporting high cell viabilities and productivity and product quality. However, given that animal cells are potentially more sensitive than microbial cells to agitation and aeration in stirred tank bioreactors, the proper design and operation of bioreactors in relation to agitation and aeration is critical to bene t fully from process optimization efforts. The perceived sensitivity of animal cells to hydrodynamic shear stresses has resulted in the use of low agitation intensities and aeration rates, which in turn can lead to inhomogeneities in dissolved oxygen and CO2 , but especially in pH at large scales of operation. The discussion in this section focuses on these issues. Although air-lift, hollow- ber, and xed bed (for anchorage-dependent cells) bioreactors are also used, the majority of industrial processes use stirred-tank bioreactors, and for that reason, the discussion here has been limited to stirred tank bioreactors. The issues related to agitation and aeration are summarized in this section, although several excellent reviews (Prokop and Bajpai, 1992; Aunins and Henzler, 1993; Wu, 1995; Joshi et al., 1996; Thomas and Zhang, 1998) on this subject have been published and the reader is referred to these for detailed discussions.

To return the first invalid item in MyList, use the following array formula:

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Use the drill gauge to measure the size of your headrest metal posts, as shown in Figure 18-11. The drill gauge is basically a measurement tool with different sized holes on it. The holes are marked so that you know the size of each hole. Poke the rod you want to measure through each hole until you find the one that is closest in size. The measurement beside the hole is the size of the rod. The metal rods on my headrest are just shy of 13 32", so I used a 13 32" drill bit for my camera mount.

5. Now, click the squares in the Conditional Text panel to the far left of the various conditions. If the square shows an eye, any text tagged with that condition will be visible. If the square is empty, any text tagged with that condition will not appear. By controlling what conditions are active (eye icons), you determine what displays and prints in your

Notice that the only thing that changes between the two queries is the PARTITION BY clause. In this version, it partitions by the zip code, to return the maximum count within the zip code. The query then returns the product groups whose counts match the maximum.

Figure 1.46 Structures of two main tetrasaccharides with a three O-glycosidic links. Cellotetraose is representative of a glycose tetrasaccharide and Stachyose of glycoside tetrasaccharide. The 3-letter codebased nomenclature is used. Arrows show chain directions as determined by the convention described in the legend to Fig. 1.45.

Notice that it does not matter whether the increment or decrement operator appears before or after the operand. But there are some cases in which this distinction is important. You should note that when using the increment and decrement operators in conjunction with an assignment operator (=), the order matters. For instance, this operator:

Column de nitions via the <colgroup> or <col> tags do not eliminate or change the necessity of td tags (which actually form the columns). You must still take care in placing your <td> tags to ensure proper data positioning within columns.

The Standards and Practices Committee of the Interactive Advertising Bureau (IAB) established a series of standard sizes for banner ads. Although no law dictates that these guidelines have to be followed, the vast majority of commercial sites adhere to the suggested dimensions. The most common banner sizes (in pixels) and their official names are listed in Table 9-2; additional banner guidelines are available at the IAB Web site (www.iab.net).

probability of occurrence (333% for each type of session) The traf c sources send data by using a unidirectional TBF connection between the mobile station and the network The signalling procedures for TBF establishments, which depend on the data source (the mobile or the network) and on the mobility management state of the mobile, explained in Section 121 Also, the measurements report messages sent for cell reselection are considered because they represent a high percentage of the load on signalling channels They are transmitted on (P)CCCH when the mobile is in the MM READY state and in packet idle mode, that is, when the mobile is not transmitting data and the READY timer has not expired The reporting periods are determined by network parameters (NC REPORTING PERIOD T for packet transfer mode and NC REPORTING PERIOD I for packet idle mode).

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